The antiserum was produced against synthesized Acetyl-peptide derived from human p57Kip2 around the Acetylation site of Lys278.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
p57Kip2 (Acetyl-Lys278) Antibody detects endogenous levels of p57Kip2 protein only when acetylated at Lys278.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDKN1C
Database Links
Gene ID: 1028 Swiss-Prot #: P49918
OMIM #: 600856 Unigene #: Hs.106070
Target Modification
Acetyl
Modification Sites
H:K278, M:K309, R:K306
Application Images
Image 1
Western blot analysis of lysate from 4T1 cells, using p57Kip2 (Acetyl-Lys278) Antibody.