The antiserum was produced against synthesized Acetyl-peptide derived from human HSP90A/B around the Acetylation site of Lys292/284.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC 1:50~100 ELISA: 1:10000
Specificity
HSP90A/B (Acetyl-Lys292/284) Antibody detects endogenous levels of HSP90A/B protein only when acetylated at Lys292/284.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HSP90AA1/HSP90AB1
Database Links
Gene ID: 3326 Swiss-Prot #: P07900/P08238
OMIM #: 140572 Unigene #: Hs.509736
Target Modification
Acetyl
Modification Sites
H:K292/284, M:K293/284, R:K293/284
Application Images
Image 1
Western blot analysis of lysate from K562 cells, using HSP90A/B (Acetyl-Lys292/284) Antibody.