The antiserum was produced against synthesized Acetyl-peptide derived from human APE1 around the Acetylation site of Lys6.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC 1:50~100 ELISA: 1:10000
Specificity
APE1 (Acetyl-Lys6) Antibody detects endogenous levels of APE1 protein only when acetylated at Lys6.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
APEX1
Database Links
Gene ID: 328 Swiss-Prot #: P27695
OMIM #: 107748 Unigene #: Hs.73722
Target Modification
Acetyl
Modification Sites
H:K6
Application Images
Image 1
Western blot analysis of lysate from A549 cells, using APE1 (Acetyl-Lys6) Antibody.