| Description: | ExoFast? Exosome Isolation Reagent from cell culture media?enables fast and efficient enrichment of intact exosomes from cell culture media samples.?Isolation of exosomes can be a tedious, non-specific, and difficult process. ExoFast? Exosome Isolation Reagent from cell culture media enables simple and reliable concentration of intact exosomes from cell media samples with a protocol that is scalable depending on your sample size. By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the cell media sample, and the solution is incubated overnight at 2°C to 8°C. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 60 min. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. |
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