NF-kappaB p65 (Acetyl-Lys310) Antibody detects endogenous levels of total NF-kappaB p65 protein only when acetylated at Lys310.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RELA
Database Links
Gene ID: 5970 Swiss-Prot #: Q04206
OMIM #: 164014 Unigene #: Hs.502875
Target Modification
Acetyl
Modification Sites
H:K310 M:K310
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 3
Western blot analysis of lysates from HeLa cells, treated with TSA 40nM 24h, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The lane on the right is blocked with the synthesized peptide.