The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys379.
Immunogen Range
336-385
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
Specificity
p53 (Acetyl-Lys379) Antibody detects endogenous levels of total p53 protein only when acetylated at Lys379.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys379) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 (Acetyl-Lys379) Antibody. The picture on the right is blocked with the synthesized peptide.