The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Histone H1 (Acetyl-Lys25) Antibody
Synonyms
H1; H1 histone family member 5; H1.5; H15; H1F5; HIST1H1B; histone 1; histone H1.5; histone H1a
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H1 around the acetylated site of Lys25.
Immunogen Range
131-180
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
Histone H1 (Acetyl-Lys25) Antibody detects endogenous levels of total Histone H1 protein only when acetylated at Lys25.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
H1FOO
Database Links
Gene ID: 132243 Swiss-Prot #: Q8IZA3
Unigene #: Hs.97358
Target Modification
Acetyl
Modification Sites
H:K25
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H1 (Acetyl-Lys25) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H1 (Acetyl-Lys25) Antibody. The lane on the right is blocked with the synthesized peptide.