Mix-n-Stain? CF750 Antibody Labeling Kit is a near-infrared fluorescent dye that is the brightest fluorescent dye available in its spectrum. Antibody conjugates prepared from CF?750 have by far the best signal-to-noise ratio than antibody conjugates prepared from other spectrally similar dyes.
Mix-n-Stain? antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your primary antibody with biotin or one of our superior CF? dyes in 30 minutes without the need for a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF?dyes are highly water soluble, small organic dyes designed for labeling proteins and nucleic acids. Many of our CF? dyes are brighter and more photostable than competing dyes. Click the link for a complete list of more than 20 GoldBio CF? antibody labeling kits.
Note: We do not recommend using CF?750 labeled primary antibodies for use with the APC-Cy7 channel. The excitation wavelength for the APC-Cy7 channel is suboptimal for CF?750 and direct immunofluorescence staining using primary antibodies labeled with CF?750 generally results in very low signal.
Technical SummaryAbs/Em Maxima: 755/777 nmExcitation laser line: 633 nm, 635 nm, 680 nm or 685 nmExtinction Coefficient: 250,000MW: ~3009 g/mol | | Kit ComponentsDyeMix-n-Stain? 10X reaction bufferMix-n-Stain? antibody storage bufferUltrafiltration vial |
Storage/Handling: Store the kit at -20°C.
Product may be shipped on room temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.