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Millipore/CBL150 | Anti-CD3 Antibody, clone UCHT1/CBL150/100 µg
  • Millipore/CBL150 | Anti-CD3 Antibody, clone UCHT1/CBL150/100 µg

Millipore/CBL150 | Anti-CD3 Antibody, clone UCHT1/CBL150/100 µg

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貨號(hào): CBL150
品牌: Millipore
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    • Description
      CatalogueNumberCBL150
      Replaces04-460
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-CD3Antibody,cloneUCHT1
      AlternateNames
      • TCR
      ProductInformation
      FormatPurified
      PresentationThemonoclonalispresentedataconcentrationof100μg/1mlinphosphatebufferedsalinecontaining10mMsodiumazideand1mg/mlbovineserumalbumin.Werecommendthateachlaboratorydetermineanoptimumworkingtitreforuseinitsparticularapplication.
      StorageandShippingInformation
      StorageConditionsForusewithin1monthofpurchasestoreat+4°C,forlongtermstoragealiquotantibodyintosmallvolumesandstoreat-20°C.
      Applications
      ApplicationAnti-CD3Antibody,cloneUCHT1detectslevelofCD3&hasbeenpublished&validatedforuseinFC,IF,IP,FUNC,IH.
      KeyApplications
      • FlowCytometry
      • Immunofluorescence
      • Immunoprecipitation
      • AffectsFunction
      • Immunohistochemistry
      ApplicationNotesIdentificationofCD3positiveTlymphocytesbyindirectimmunofluorescencestaining

      Leukaemiaandlymphomaphenotyping

      IdentificationofnormalandmalignantTlymphocytesinfrozentissuesections

      ImmunoprecipitationoftheCD3antigen

      MitogenicstimulationofTlymphocyteswithorwithoutthepresenceofIL-2

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenPurifiedhumanTcells
      CloneUCHT1
      HostMouse
      SpecificityThisantibodyrecognizes22/26/30kDamolecularweightlymphocytesurfacemoleculesassociatedwiththeTcellantigenreceptorcomplex.CD3iscloselyassociatedonthelymphocytecellsurfacewiththeTcellantigenreceptorTcR(2-4).ItisbelievedthattheCD3complextransmitsactivationalsignalstotheTcell"sinterioruponantigenrecognitionbyTcR.CD45(LCA),aproteintyrosinephosphatase,isapotentregulatorofsignaltransductionbytheCD3complex.ItreactswiththemajorityofperipheralbloodTlymphocytes,amajorproportionofthymocytes,themajorityofTcellchroniclymphocyticleukaemiacells,Sézaryleukaemiasandapproximately70%ofacutelymphoblasticleukaemiasofTcellorigin.
      Antigendistribution:
      Tcells100%
      PeripheralBloodlymphocytes75+7%
      Thymocytes68+5%
      Granulocytes<>
      Monocytes<>
      Bcells(E-)<>
      NKcells(CD16+)<>
      KNOWNSPECIESCROSSREACTIVITY:Negativewithrhesusmonkeyperipheralbloodleucocytesinflowcytometry
      FUSIONPARTNER:P3x63Ag8.653myelomacellline
      IsotypeIgG1
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneistheCD3-epsilonpolypeptide,whichtogetherwithCD3-gamma,-deltaand-zeta,andtheT-cellreceptoralpha/betaandgamma/deltaheterodimers,formstheT-cellreceptor-CD3complex.Thiscomplexplaysanimportantroleincouplingantigenrecognitiontoseveralintracellularsignal-transductionpathways.Thegenesencodingtheepsilon,gammaanddeltapolypeptidesarelocatedinthesameclusteronchromosome11.TheepsilonpolypeptideplaysanessentialroleinT-celldevelopment.Defectsinthisgenecauseimmunodeficiency.ThisgenehasalsobeenlinkedtoasusceptibilitytotypeIdiabetesinwomen.
      GeneSymbol
      • CD3E
      • T3E
      • TCRE
      Non-ReactiveSpecies
      • Monkey
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P07766#TheCD3complexmediatessignaltransduction.
      SIZE:207aminoacids;23147Da
      SUBUNIT:TheTCR/CD3complexofT-lymphocytesconsistsofeitheraTCRalpha/betaorTCRgamma/deltaheterodimercoexpressedatthecellsurfacewiththeinvariantsubunitsofCD3labeledgamma,delta,epsilon,zeta,andeta.
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      SIMILARITY:SwissProt:P07766##Contains1Ig-like(immunoglobulin-like)domain.&Contains1ITAMdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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