Synonym:CollagenType4Properties:
RelatedCategories | ApplicationIndex,AttachmentFactors,BiochemicalsandReagents,CellCulture,ClinicalChemistry, |
description | SigmaTypeVI |
form | powder |
impurities | HIV,hepatitisBandhepatitisC,nonedetected |
solubility | 0.5Maceticacid:1 mg/mL |
suitABIlity | suitableforsubstrateforCollagenase |
FeaturedIndustry | DiagnosticAssayManufacturing |
storagetemp. | −20°C |
GeneInformation | human...COL4A2(1282),COL4A2(1284),COL4A3(1285),COL4A4(1286),COL4A5(1287),COL4A6(1288) |
Description:
Components
Allcollagenmoleculesarecomposedofthreepolypeptidechainsarrangedinatriplehelicalconformation,withaprimarystructurethatismostlyarepeatingmotifwithglycineineverythirdpositionandprolineor4-hydroxyprolinefrequentlyprecedingtheglycineresidue.TypeIVcollagenoccursonlyinthebasementmembranesandcontainsuptosixgeneticallydistincta-chains.
AnalysisNote
AnSDSpolyacrylamidegelelectrophoresistestrununderreducingconditionsconsistentwithbasementmembranecollagenyieldsthreemajorbands.
OtherNotes
Collagenisclassifiedintoanumberofstructurallyandgeneticallydistincttypes.WeusethenomenclatureproposedbyBornsteinandTraub.BewaryofconfusingSigma-typedesignationswithrecognizedcollagenclassificationtypes.
Packaging
10,50mginpolybottle
5mginglassbottle
PreparationNote
Thisisalyophilizedpowderthatcanbereconstitutedinsterile.5Maceticacid,PBSorwaterat1mg/mL.APBSsolutionwillbestableforatleastoneyearat-20°C.ItispreparedbyamodificationofthepepsinextractionmethodofGlanville,R.W.,etal.
Biochem/physiolActions
Duringdevelopment,collagenIVisubiquitouslydistributedinBMs.Duringthematurationprocess,thisnetworkgetspartiallyreplacedinaremarkablytissuespecificmanner,definingBMstructureandfunction.CollagenIVhasbeenshowntobindtoplatelets,hepatocytes,keratinocytes,endothelial,mesangial,pancreaticcellsandsometumorcells.
Safety:
PersonalProtectiveEquipment
Eyeshields,Gloves,typeN95(US),typeP1(EN143)respiratorfilter
RIDADR
NONHforallmodesoftransport