Synonym:CarbonateDehydratase,CarbonateHydrolyaseProperties:
RelatedCategories | 4.2.x.xC-Obonds,4.x.x.xLyases,AnalyticalandIndustrialEnzymes,ApplicationIndex,BiochemicalsandReagents, |
form | lyophilizedpowder |
FeaturedIndustry | DiagnosticAssayManufacturing |
storagetemp. | 2-8°C |
Description:
FrequentlyAskedQuestions
FrequentlyAskedQuestionsareavailableforthisProduct.
Packaging
100,500mginpolybottle
UnitDefinition
OneWilbur-Anderson(W-A)unitwillcausethepHofa0.02MTrizmabuffertodropfrom8.3to6.3perminat0°C.(OneW-AunitisessentiallyequivalenttooneRoughton-Boothunit.)
Application
Carbonicanhydrasefrombovineerythrocytes(BCA)hasbeenusedtostudytheeffectofremovalofenzyme-boundmetalion,Zn2+,onaggregationbehavioroftheenzyme.RemovalofmetalionbyachelatorsuchasEDTAenhancesthepropensityoftheenzymetoadoptthemolten-globulestate.Thisstateoftheenzymeisfoundtobindtothechaperone-likeα-crystallinandpreventaggregation.TheenzymefromSigmahasbeenimmobilizedtoelectRochemicaltransducersinordertoobtaindevelopananalyticaldevicefordissolvedCO2measurement.IthasalsobeenusedfortheThermodynamicanalysisofconformationalchangesinBCA.
Biochem/physiolActions
Carbonicanhydraseisazincmetalloenzymethathasamolecularweightofapproximately30,000Da.Theenzymecatalyzesthehydrationofcarbondioxidetocarbonicacid.ItisinvolvedinvitalphysiologicalandpathologicalprocessessuchaspHandCO2homeostasis,transportofbicarbonateandCO2,biosyntheticreactions,boneresorption,calcification,andtumOrigenicity.Therefore,thisenzymeisanimportanttargetforinhibitorswithclinicalapplicationsforvariouspathologiessuchasglaucoma,epilepsyandParkinson’sdisease.
Safety:
PersonalProtectiveEquipment
Eyeshields,Gloves,typeN95(US),typeP1(EN143)respiratorfilter
RIDADR
NONHforallmodesoftransport