The antiserum was produced against synthesized peptide derived from human HSP27.
Immunogen Range
May-54
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
HSP27 Antibody detects endogenous levels of total HSP27 protein.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HSPB1
Database Links
Gene ID: 3315 Swiss-Prot #: P04792
OMIM #: 602195/606595/608634 Unigene #: Hs.520973
Target Modification
Unmodified/Total
Modification Sites
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSP27 Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from HeLa cells, using HSP27 Antibody. The lane on the right is blocked with the synthesized peptide.