JOEisafluorescentdyewiththeemissioninyellowregionofthespectrum.ThefluorophorematcheschannelofHEXdye,anotherchlorinatedfluorescein.
JOEdyeisoftenusedinqPCR;oligolabelingcanbeachievedusingClickchemistry.ThisisanazidederivativeofJOEforClickchemistry,pure5-isomer.
Recommendedprotocol
Click-ChemistryLabelingofOligonucleotidesandDNA
Generalproperties
Appearance: | redsolid |
MassspecM+increment: | 586.1 |
Molecularweight: | 587.37 |
Molecularformula: | C26H20N4Cl2O8 |
Solubility: | goodinDMF,DMSO |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |