The antiserum was produced against synthesized peptide derived from human Akt.
Immunogen Range
212-261
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Akt Antibody detects endogenous levels of total Akt protein.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AKT1
Database Links
Gene ID: 207 Swiss-Prot #: P31749
OMIM #: 164730 Unigene #: Hs.525622
Target Modification
Unmodified/Total
Modification Sites
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from HeLa cells, treated with Etoposide 25uM 24h, using Akt Antibody. The lane on the right is blocked with the synthesized peptide.