Bluo-GalisusedasanalternativetoX-Gal,andproducesadarkerbluecolor.Bluo-Galishydrolyzedbyβ-galactosidasetoproduceablueprecipitate.Thisprecipitateisinsolubleinalcoholandxylenes,thusmakingitsuitableforimmunoblottingandimmunocytochemicalassays.Bacterialcoloniescontainingactiveβ-galactosidaseproducebluecolonieswhengrownonplatescontainingBluo-Gal.
Bluo-GaliscommonlyusedinconjunctionwithIPTG(GoldBio#I2481)forblue-whitescreening.
ProductSpecifications:
Bluo-Gal
5-Bromo-3-indolyl-β-D-galactopyranoside
Blue-Gal,Lapis™-β-D-Gal
MOLECULARBIOLOGYGRADE
Formula:C14H16BrNO6
MW:374.18g/mol
Purity:>99%byHPLC
Solubleindimethylformamide(DMF)
Storage/Handling:Storeat-15°C,andprotectfromlight.
PubChemChemicalID:44161406