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Millipore/AB5669 | Anti-Spinophilin Antibody/AB5669/100 µg
  • Millipore/AB5669 | Anti-Spinophilin Antibody/AB5669/100 µg

Millipore/AB5669 | Anti-Spinophilin Antibody/AB5669/100 µg

價格: ¥4188.00 市場價: 6980.00

貨號: AB5669
品牌: Millipore
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    • Description
      CatalogueNumberAB5669
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-SpinophilinAntibody
      AlternateNames
      ProductInformation
      FormatAffinityPurified
      PresentationAffinitypurifiedimmunoglobulin.Liquidin0.02Mphosphatebuffercontaining0.25MNaCl,pH7.6with0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°Cinundilutedforupto6monthsafterdateofreceipt.
      Applications
      ApplicationAnti-SpinophilinAntibodydetectslevelofSpinophilin&hasbeenpublished&validatedforuseinWB.
      KeyApplications
      • WesternBlotting
      ApplicationNotesWesternblot:0.05-0.1μg/mLusingECLonratbrainlysate.Reactswithabandof~140kDa.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidefromratSpinophilin.
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificitySpinophilin
      SpeciesReactivity
      • Rat
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummarySpinophilinisaregulatorysubunitofproteinphosphatase-1catalyticsubunit(PP1;seeMIM176875)andishighlyenrichedindendriticspines,specializedprotrusionsfromdendriticshaftsthatreceivemostoftheexcitatoryinputinthecentralnervoussystem(Allenetal.,1997[PubMed9275233]).[suppliedbyOMIM]
      GeneSymbol
      • PPP1R9B
      • Spinophilin
      • PPP1R9
      • Neurabin-2
      • PPP1R6
      • FLJ30345
      • spinophilin
      • Neurabin-II
      • SPINO
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q96SB3#Seemstoactasascaffoldproteininmultiplesignalingpathways.Modulatesexcitatorysynaptictransmissionanddendriticspinemorphology.Bindstoactinfilaments(F-actin)andshowscross-linkingactivity.BindsalongthesidesoftheF-actin.MayplayanimportantroleinlinkingtheactinCytoskeletontotheplasmamembraneatthesynapticjunction.Believedtotargetproteinphosphatase1/PP1todendriticspines,whicharerichinF-actin,andregulatesitsspecificitytowardionchannelsandothersubstrates,suchasAMPA-typeandNMDA-typeglutamatereceptors.PlaysaroleinregulationofG-proteincoupledreceptorsignaling,includingdopamineD2receptorsandalpha-adrenergicreceptors.MayestablishasignalingcomplexfordopaminergicneurotransmissionthroughD2receptorsbylinkingreceptorsdownstreamsignalingmoleculesandtheactincytoskeleton.BindstoADRA1BandRGS2andmediatesregulationofADRA1Bsignaling.MayconfertoRacsignalingspecificitybybindingtoboth,RacGEFsandRaceffectorproteins.Probablyregulatesp70S6kinaseactivitybyformingacomplexwithTIAM1(Bysimilarity).


      SIZE:815aminoacids;89192Da

      SUBUNIT:PossIBLyexistsasanhomodimer,homotrimeroranhomotetramer.InteractswithF-actin,PPP1CA,neurabin-1,TGN38andD(2)dopaminereceptor.InteractswithRGS1,RGS2,RGS4,RGS19andADRA1B,ADRA2A,ADRA2B,ADRA2C,CDKN2A,PPP1R2,RASGFR1andTIAM1.

      SUBCELLULARLOCATION:Cytoplasm(Bysimilarity).Nucleus(Bysimilarity).Cellprojection,dendriticspine(Bysimilarity).Celljunction,synapse.Note=Enrichedatsynapseandcadherin-basedcell-celladhesionsites.Inneurons,bothcytosolicandmembrane-associated,andhighlyenrichedinthepost-synapticdensityapposedtoexitatorysynapses.ColocalizeswithPPP1R2atactin-richadherensjunctionsinepithelialcellsandindendriticspines(Bysimilarity).




      PTM:StimulationofD1(butnotD2)dopaminereceptorsinducesSer-94phosphorylation.DephosphorylationofSer-94ismediatedmainlybyPP1andtoalesserextentbyPP2A.PhosphorylationofspinophilindisruptsitsassociationwithF-actin,butdoesnotaffectitsbindingtoPP1(Bysimilarity).


      SIMILARITY:SwissProt:Q96SB3##Contains1PDZ(DHR)domain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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