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Millipore/AB5600-50UL | Anti-Synaptotagmin I Antibody, luminal part/AB5600-50UL/50 µL
  • Millipore/AB5600-50UL | Anti-Synaptotagmin I Antibody, luminal part/AB5600-50UL/50 µL

Millipore/AB5600-50UL | Anti-Synaptotagmin I Antibody, luminal part/AB5600-50UL/50 µL

價(jià)格: ¥3696.00 市場(chǎng)價(jià): 6160.00

貨號(hào): AB5600-50UL
品牌: Millipore
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    • Description
      CatalogueNumberAB5600-50UL
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-SynaptotagminIAntibody,luminalpart
      AlternateNames
      • p65
      • Syt1
      ProductInformation
      FormatAffinityPurified
      Control
      • CONTROLANTIGEN:Includedfreeofchargewiththeantibodyis20μgofcontrolantigen(lyophilizedpowder).Thestocksolutionoftheantigencanbemadeupusing100μLofsteriledeionizedwater.Fornegativecontrol,preincubate1μgofpeptidewith1μgofantibodyforonehouratroomtemperature.Optimalconcentrationsmustbedeterminedbytheenduser.
      PresentationAffinitypurifiedimmunoglobulin.Lyophilizedfromphosphatebufferedsaline,pH7.4,containing1%BSA,5%sucroseasastABIlizerand0.025%sodiumazideasapreservative.Reconstitutewith50μLofsteriledeionizedwater.Centrifugeantibodypreparationbeforeuse(10,000xgfor5min).
      StorageandShippingInformation
      StorageConditionsMaintainlyophilizedmaterialat-20°Cforupto12months.Afterreconstitutionmaintainat-20°Cinundilutedaliquotsforupto6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationDetectSynaptotagminIusingthisAnti-SynaptotagminIAntibody,luminalpartvalidatedforuseinWB,IH.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesWesternblot:1:200-1:1,000usingECLonratbrainmembranes.

      Immunohistochemistryonratbrainsections.

      DilutionsshouldbemadeusingacarrierproteinsuchasBSA(1-3%)

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenPeptidecorrespondingtoaminoacids1-19fromratSynaptotagminI(Accession#P21107).
      Epitopeluminalpart
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityRecognizesSynaptotagminI,luminalpart.Theepitopedoesnotsharehomologywithanyotherknownproteins.

      SPECIESREACTIVITIES:Itisexpectedthattheantibodywillalsorecognizehuman.Otherspecieshavenotbeentested.
      SpeciesReactivity
      • Rat
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThesynaptotagminsareintegralmembraneproteinsofsynapticvesiclesthoughttoserveasCa(2+)sensorsintheprocessofvesiculartraffickingandexocytosis.CalciumbindingtosynaptotagminIparticipatesintriggeringneurotransmitterreleaseatthesynapse(Fernandez-Chaconetal.,2001[PubMed11242035]).[suppliedbyOMIM]
      GeneSymbol
      • SYT1
      • DKFZp781D2042
      • SVP65
      • SytI
      • SYT
      • p65
      • P65
      • Synaptotagmin-1
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P21579#Mayhavearegulatoryroleinthemembraneinteractionsduringtraffickingofsynapticvesiclesattheactivezoneofthesynapse.Itbindsacidicphospholipidswithaspecificitythatrequiresthepresenceofbothanacidicheadgroupandadiacylbackbone.ACa(2+)-dependentinteractionbetweensynaptotagminandputativereceptorsforactivatedproteinkinaseChasalsobeenreported.ItcanbindtoatleastthreeadditionalproteinsinaCa(2+)-independentmanner;theseareneurexins,syntaxinandAP2.
      COFACTOR:Binds3calciumionspersubunit.TheionsareboundtotheC2domains(Bysimilarity).
      SIZE:422aminoacids;47573Da
      SUBUNIT:Homotetramer(Probable).InteractswithSTN2,SV2A,SV2B,SV2CandRIMS1.FormsacomplexwithSV2B,syntaxin1andSNAP25(Bysimilarity).
      SUBCELLULARLOCATION:Cytoplasmicvesicle,secretoryvesicle,synapticvesicle.Cytoplasmicvesicle,secretoryvesicle,chromaffingranule.Note=Synapticvesiclesandchromaffingranules.
      DOMAIN:SwissProt:P21579ThefirstC2domainmediatesCa(2+)-dependentphospholipidbinding.&ThesecondC2domainmediatesinteractionwithSV2AandSTN2(Bysimilarity).
      SIMILARITY:Belongstothesynaptotagminfamily.&Contains2C2domains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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