Immunogen | SyntheticpeptidefromhumanSemaphorin3F(CatalognumberAG363).(TPWTQTQAVRFRFSRATDGALRPM). |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | RecognizesSemaphorin3F(Sema3F),recombinantandpurifiedprotein.ByWesternblottheantibodyrecognizesaproteinof90kDaintotalextractsfromtissueculturecellsproducingSema3F.Semaphorin3Fisexpressedubiquitouslywithhigherlevelsinlung,brain,spleen,muscleandheart. |
SpeciesReactivity | |
SpeciesReactivityNote | ThisantibodymayalsoreactwithmousebecauseofextremelyhighsequenceconservationintheSEM3Fprotein. |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thesemaphorinsareafamilyofproteinsthatareinvolvedinsignaling.Allthefamilymembershaveasecretionsignal,a500-aminoacidsemadomain,and16conservedcysteineresidues(Kolodkinetal.,1993[PubMed8269517]).Sequencecomparisonshavegroupedthesecretedsemaphorinsinto3generalclasses,allofwhichalsohaveanimmunoglobulindomain.ThesemaphorinIIIfamily,consistingofhumansemaphorinIII(SEMA3A;MIM603961),chickencollapsin,andmousesemaphorinsA,D,andE,allhaveabasicdomainattheCterminus.Chickencollapsincontributestopathfindingbyaxonsduringdevelopmentbyinhibitingextensionofgrowthcones(Luoetal.,1993[PubMed8402908])throughaninteractionwithacollapsinresponsemediatorproteinofrelativemolecularmass62K(CRMP62)(Goshimaetal.,1995[PubMed7637782]),aputativehomologofanaxonalguidanceassociatedUNC33geneproduct(MIM601168).SEMA3FisasecretedmemberofthesemaphorinIIIfamily.[suppliedbyOMIM] |
GeneSymbol | - SEMA3F
- SEMAK
- SEMA-IV
- SEMA4
- Sema4
- sema4
|
PurificationMethod | ImmunoAffinityPurified |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:Q13275#Mayplayaroleincellmotilityandcelladhesion. SIZE:785aminoacids;88381Da SUBCELLULARLOCATION:Secreted(Bysimilarity). TISSUESPECIFICITY:Expressedabundantlybutdifferentiallyinavarietyofneuralandnonneuraltissues.Thereishighexpressioninmammarygland,kidney,fetalbrain,andlungandlowerexpressioninheartandliver. DEVELOPMENTALSTAGE:Detectedasearlyasembryonicday10. SIMILARITY:SwissProt:Q13275##Belongstothesemaphorinfamily.&Contains1Ig-likeC2-type(immunoglobulin-like)domain.&Contains1Semadomain. |
MolecularWeight | 90kDa |