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Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg
  • Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg

Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg

價格: ￥4068.00 市場價: 6780.00
貨號: AB3756
品牌: Millipore
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    • Description
      CatalogueNumberAB3756
      ReplacesMABE515
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Rad51Antibody
      ProductInformation
      FormatAffinityPurified
      PresentationAffinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainunopenedvialat-70Cforupto6months.Avoidrepeatedfreeze/thawcycles.

      Preparation&Use:Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnoteffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

      ResuspendtheantibodypelletinanysuitableBIOLOGicalbuffer,standardPBSorTBS(pH7.3-7.5)aretypical.Volumesrequiredarenotcriticalbutitissuggestedthatthefinalantibodyconcentrationbebetween0.1mg/mLand1.0mg/mL.Forexample,toachievea1mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL.

      Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25C°priortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

      Therehydratedantibodysolutionscanbestoredundilutedat2-8C°for2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8C°.Forstorageat-20C°,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.Forfreezing,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70C°forupto6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationDetectRad51withAnti-Rad51Antibody(RabbitPolyclonalAntibody),thathasbeenshowntoworkinWB,ICC&IHC.
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      • Immunohistochemistry
      ApplicationNotesWesternblot:1:200-1:1,000usingECL.

      Immunohistochemistry:1:200-1:1,000.

      Immunocytochemistry:1:200-1:1,000.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      SUGGESTEDPROTOCOLFORIMMUNOHISTOCHEMISTRY

      1.Dissecttissuesandfreezeondryice

      2.Cutonacryostat-10umslices

      3.Dryslidesatroomtemperaturefor30-90min

      4.FixwithcoldAcetone/Methanol(50/50)2min-theneitherprocessfurtherorstoreat-20°C.Alternativeisfixationwith4%PFAandtreatmentwithatrypsinsolution.(0.05%trypsin,incubation10minutesat37°C,followedby3washeswithPBS)

      5.Letairdry

      6.PBS5min

      7.Incubatein50mMammoniumchloride30min

      8.PBS5min

      9.Blockingserum30-45min

      10.Primaryantibody90min-dilution1:100-1:600

      11.Wash3timesfor5min

      12.Secondaryfluorescentconjug.antibody30min

      13.Wash3timesfor5minandmount
      BiologicalInformation
      ImmunogenSyntheticpeptide,aminoacids25-41ofRAD51.
      HostRabbit
      SpecificityRecognizesRAD51.Themolecularweightoftheproteinis36,963Daltons.
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheRAD51proteinfamily.RAD51familymembersarehighlysimilartobacterialRecAandSaccharomycescerevisiaeRad51,andareknowntobeinvolvedinthehomologousrecombinationandrepairofDNA.ThisproteincaninteractwiththessDNA-bindingproteinRPAandRAD52,anditisthoughttoplayrolesinhomologouspairingandstrandtransferofDNA.ThisproteinisalsofoundtointeractwithBRCA1andBRCA2,whichmaybeimportantforthecellularresponsetoDNAdamage.BRCA2isshowntoregulateboththeintracellularlocalizationandDNA-bindingABIlityofthisprotein.LossofthesecontrolsfollowingBRCA2inactivationmaybeakeyeventleADIngtogenomicinstabilityandtumOrigenesis.Twoalternativelysplicedtranscriptvariantsofthisgene,whichencodedistinctproteins,havebeenreported.TranscriptvariantsutilizingalternativepolyAsignalsexist.
      GeneSymbol
      • RAD51
      • RAD51L3
      • HsT16930
      • BRCC5
      • hRAD51
      • HRAD51
      • RECA
      • HsRad51
      • RAD51D
      • HsRAD51
      • RAD51A
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q06609#MayparticipateinacommonDNAdamageresponsepathwayassociatedwiththeactivationofhomologousrecombinationanddouble-strandbreakrepair.BindstosingleanddoublestrandedDNAandexhibitsDNA-dependentATPaseactivity.UnderwindsduplexDNAandformshelicalnucleoproteinfilaments.
      SIZE:339aminoacids;36966Da
      SUBUNIT:InteractswithBRCA1,BRCA2andeitherdirectlyorindirectlywithp53.InteractswithXRCC3,RAD54LandRAD54B.PartofacomplexwithRAD51CandRAD51B.InteractswithRAD51AP1.InteractswithCHEK1/CHK1,andthismayrequirepriorphosphorylationofCHEK1.
      SUBCELLULARLOCATION:Nucleus.Note=ColocalizeswithRAD51AP1tomultiplenuclearfociuponinductionofDNAdamage.
      TISSUESPECIFICITY:Highlyexpressedintestisandthymus,followedbysmallintestine,placenta,colon,pancreasandovary.Weaklyexpressedinbreast.
      PTM:Phosphorylated.PhosphorylationofThr-309byCHEK1/CHK1mayenhanceassociationwithchromatinatsitesofDNAdamageandpromoteDNArepairbyhomologousrecombination.DISEASE:SwissProt:Q06609#DefectsinRAD51areassociatedwithbreastcancer(BC)[MIM:114480].
      SIMILARITY:SwissProt:Q06609##BelongstotherecAfamily.RAD51subfamily.&Contains1HhHdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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