Immunogen | Syntheticpeptidecorrespondingtoaminoacids100-116ofmurinecaspase-12. |
Epitope | prodomain,aa100-116 |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | Antibodyrecognizescaspase-12pro-proteininmouseextractsofnormalandapoptoticcells.Caspase-12ispredictedtohavealarge,>120aa,longprodomainregion.Thusthisantibodyrecognizesonlytheproformsoftheenzymeandnottheactivesubunits.Uncleaved(proform)caspase-12isprominentlydetectedinmanytissues.Activeformsaredifficulttodetectexpectintransfectcellsystems.{Kalai,Metal(2003)162:457-467}. |
Isotype | IgG |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneSummary | CaspasesarecysteineproteasesthatcleaveC-terminalasparticacidresiduesontheirsubstratemolecules.Thisgene,CASP12,ismosthighlyrelatedtomembersoftheICEsubfamilyofcaspasesthatprocessinflammatorycytokines.Inrodents,thehomologofthisgenemediatesapoptosisinresponsetoendoplasmicreticulumstress.However,inhumansthisgenecontainsapolymorphismforthepresenceorabsenceofaprematurestopcodon.Themajorityofhumanindividualshavetheprematurestopcodonandproduceatruncatednon-functionalprotein.Theread-throughcodonoccursprimarilyinindividualsofAfricandescentandcarriershaveendotoxinhypo-responsivenessandanincreasedsusceptibilitytoseveresepsis. |
GeneSymbol | - CASP12
- Caspase-12.
- CASP12P1
|
PurificationMethod | ImmunoAffinityPurified |
UniProtNumber | |
UniProtSummary | SIZE:341aminoacids;38907Da |