Immunogen | AsyntheticpeptidefromtheC-terminalheptadeapeptideofmouseSNAP-25(correspondingtoresiduesCys+190-206:[C]RIDEANQRATKMLGSG). |
Host | Rabbit |
Specificity | SNAP-25(synaptosome-associatedproteinof25kDa)isaSNAP(solubleN-ethylmaleimide-sensitivefusion(NSF)attachmentprotein)receptor(SNARE-relatedprotein)whichistightlyassociatedwiththeplasmamembranebymeansofthepalmitoylationofaclustercysteinresidueswithitsstructure.NocrossreactivitywithSNAP-23isexpected.
SNAP-25isspecificallycleavedclosetothecarboxylterminalbythebotulinumneurotoxins(BoNT)AandEwhichareknowntoinhibitneurotransmitterrelease.SimilarinhibitoryeffectsofBoNT/AandEhavebeenrecordedfornon-neuronalsecretorycells,suchasadrenalmedulla,andthisleadtotheidentificationofSNAP-25inSNARE-likecomplexesprecipitatedfromchromaffincells. |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | SynapticvesiclemembranedockingandfusionismediatedbySNAREs(solubleN-ethylmaleimide-sensitivefactorattachmentproteinreceptors)locatedonthevesiclemembrane(v-SNAREs)andthetargetmembrane(t-SNAREs).Theassembledv-SNARE/t-SNAREcomplexconsistsofabundleoffourhelices,oneofwhichissuppliedbyv-SNAREandtheotherthreebyt-SNARE.Fort-SNAREsontheplasmamembrane,theproteinsyntaxinsuppliesonehelixandtheproteinencodedbythisgenecontributestheothertwo.Therefore,thisgeneproductisapresynapticplasmamembraneproteininvolvedintheregulationofneurotransmitterrelease.Twoalternativetranscriptvariantsencodingdifferentproteinisoformshavebeendescribedforthisgene. |
GeneSymbol | - SNAP25
- SNAP
- dJ1068F16.2
- SNAP-25
- FLJ23079
- RIC-4
- SUP
- SEC9
- RIC4
- bA416N4.2
|
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P60880#t-SNAREinvolvedinthemolecularregulationofneurotransmitterrelease.Mayplayanimportantroleinthesynapticfunctionofspecificneuronalsystems.Associateswithproteinsinvolvedinvesicledockingandmembranefusion. SIZE:206aminoacids;23315Da SUBUNIT:PartoftheSNAREcorecomplexcontainingSNAP25,VAMP2andSTX1A.ThiscomplexbindsCPLX1.InteractswithTRIM9,RIMS1,SNAP25BPandHGS.BindsSTXBP6.FoundinaternarycomplexwithSTX1AandVAMP8.FoundinacomplexcontainingSYT1,SV2Bandsyntaxin-1(Bysimilarity). TISSUESPECIFICITY:Neuronsoftheneocortex,hippocampus,piriformcortex,anteriorthalamicnuclei,pontinenuclei,andgranulecellsofthecerebellum. PTM:Palmitoylated(Bysimilarity). SIMILARITY:SwissProt:P60880##BelongstotheSNAP-25family.&Contains2t-SNAREcoiled-coilhomologydomains. |