Immunogen | SyntheticpeptidecorrespondingtotheC-terminusofthepredictedratVAChTprotein. |
Host | GuineaPig |
Specificity | VesicularAcetylcholineTransporter(VAChT).ByWesternblottheantibodyreactedwithasinglebandinratbrainhomogenates.ThisbandcorrespondedtothesizeofthepredictedrVAChT.Therewasnostainingofblotsincubatedwiththeantibodythathadbeenpreabsorbedwiththepeptideusedforantibodyproduction.Byimmunohistochemistrythestainingwasstrongintheinnerretinawherecholinergicprocessesarelocatedandweakincholinergiccellbodies.Therewasnobackgroundstainingnoted.IntheCNSstainingwasstronginfibersandstainscholinergiccellbodieswell. |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneisamemberofthevesicularaminetransporterfamily.Theencodedtransmembraneproteintransportsacetylcholineintosecretoryvesiclesforreleaseintotheextracellularspace.AcetylcholinetransportutilizesaprotongrADIentestablishedbyavacuolarATPase.Thisgeneislocatedwithinthefirstintronofthecholineacetyltransferasegene. |
GeneSymbol | - SLC18A3
- MGC12716
- VACHT
- VAChT
|
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:Q16572#Involvedinacetylcholinetransportintosynapticvesicles. SIZE:532aminoacids;56961Da SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein. TISSUESPECIFICITY:Peripheralandcentralcholinergicnervoussystems. SIMILARITY:SwissProt:Q16572##Belongstothemajorfacilitatorsuperfamily.Vesiculartransporterfamily. |