Immunogen | Nativeenzymefrombovineadrenalglands. |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | RecognizesDopaminebetahydroxylase.AB1585reactswithasinglebandonWesternblotsofbovineadrenalhomogenatesandstainsonlycellsknowntocontainDBH,suchassympatheticneurons,adrenalmedullarycellsandcentraladrenergicneurons.Stainingisabolishedbypreincubationwithenzyme. |
SpeciesReactivity | - Bovine
- GuineaPig
- Human
- Rat
- Rabbit
- Feline
|
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | TheproteinencodedbythisgeneisanoxidoreductasebelongingtothecoppertypeII,ascorbate-dependentmonooxygenasefamily.Itispresentinthesynapticvesiclesofpostganglionicsympatheticneuronsandconvertsdopaminetonorepinephrine.Itexistsinbothsolubleandmembrane-boundforms,dependingontheabsenceorpresence,respectively,ofasignalpeptide. |
GeneSymbol | - DBH
- DBM
- EC1.14.17.1[Contains:Solubledopaminebeta-hydroxylase].
|
PurificationMethod | Unpurified |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P09172#Conversionofdopaminetonoradrenaline. COFACTOR:Binds1PQQpersubunit.&Binds2copperionspersubunit. SIZE:617aminoacids;69065Da SUBUNIT:Homotetramercomposedoftwonon-covalentlybounddisulfide-linkeddimers. SUBCELLULARLOCATION:Solubledopaminebeta-hydroxylase:Cytoplasmicvesicle,secretoryvesiclelumen.Cytoplasmicvesicle,secretoryvesicle,chromaffingranulelumen.&Cytoplasmicvesicle,secretoryvesiclemembrane;Single-passtypeIImembraneprotein.Cytoplasmicvesicle,secretoryvesicle,chromaffingranulemembrane;Single-passtypeIImembraneprotein(Potential). DISEASE:SwissProt:P09172#DefectsinDBHarethecauseofDBHdeficiency[MIM:223360];alsocallednorepinephrinedeficiencyornoradrenalinedeficiency.Thisdisorderischaracterizedbyprofounddeficitsinautonomicandcardiovascularfunction,butapparentlyonlysubtlesigns,ifany,ofcentralnervoussystemdysfunction. SIMILARITY:SwissProt:P09172##BelongstothecoppertypeIIascorbate-dependentmonooxygenasefamily.&Contains1DOMONdomain. |
MolecularWeight | 69kDa |