The antiserum was produced against synthesized peptide derived from human PLD2 around the phosphorylation site of Tyr169.
Immunogen Range
136-185
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
PLD2 (Phospho-Tyr169) Antibody detects endogenous levels of PLD2 only when phosphorylated at Tyr169.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PLD2
Database Links
Gene ID: 5338 Swiss-Prot #: O14939
OMIM #: 602384 Unigene #: Hs.104519
Target Modification
Phospho
Modification Sites
H:Y169 M:Y169 R:Y169
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PLD2 (Phospho-Tyr169) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30', using PLD2 (Phospho-Tyr169) Antibody. The lane on the right is blocked with the phospho peptide.