The antiserum was produced against synthesized peptide derived from human MDM4 around the phosphorylation site of Ser367.
Immunogen Range
336-385
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
MDM4 (Phospho-Ser367) Antibody detects endogenous levels of MDM4 only when phosphorylated at Ser367.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MDM4
Database Links
Gene ID: 4194 Swiss-Prot #: O15151
OMIM #: 602704 Unigene #: Hs.658187
Target Modification
Phospho
Modification Sites
H:S367 M:S367 R:S368
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MDM4 (Phospho-Ser367) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MDM4 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.