The antiserum was produced against synthesized peptide derived from human Claudin 3 around the phosphorylation site of Tyr219.
Immunogen Range
171-220
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
Claudin 3 (Phospho-Tyr219) Antibody detects endogenous levels of Claudin 3 only when phosphorylated at Tyr219.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CLDN3
Database Links
Gene ID: 1365 Swiss-Prot #: O15551
OMIM #: 602910 Unigene #: Hs.647023
Target Modification
Phospho
Modification Sites
H:Y219
Application Images
Image 1
Western blot analysis of lysates from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right is blocked with the phospho peptide.