The antiserum was produced against synthesized peptide derived from human B-Raf around the phosphorylation site of Thr753.
Immunogen Range
717-766
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
B-Raf (Phospho-Thr753) Antibody detects endogenous levels of B-Raf only when phosphorylated at Thr753.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using B-Raf (Phospho-Thr753) Antibody. The lane on the right is blocked with the phospho peptide.