The antiserum was produced against synthesized peptide derived from human Bax around the phosphorylation site of Ser184.
Immunogen Range
143-192
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Bax (Phospho-Ser184) Antibody detects endogenous levels of Bax only when phosphorylated at Ser184.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BAX
Database Links
Gene ID: 581 Swiss-Prot #: Q07812
OMIM #: 600040 Unigene #: Hs.433670/Hs.631546
Target Modification
Phospho
Modification Sites
H:S184 M:S184 R:S184
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Bax (Phospho-Ser184) Antibody. The picture on the right is blocked with the phospho peptide.