The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr216.
Immunogen Range
181-230
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Src (Phospho-Tyr216) Antibody detects endogenous levels of Src only when phosphorylated at Tyr216.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SRC
Database Links
Gene ID: 6714 Swiss-Prot #: P12931
OMIM #: 190090 Unigene #: Hs.195659
Target Modification
Phospho
Modification Sites
H:Y216 M:Y221 R:Y216
Application Images
Image 1
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 5', using Src (Phospho-Tyr216) Antibody. The lane on the right is blocked with the phospho peptide.