The antiserum was produced against synthesized peptide derived from human BL-CAM around the phosphorylation site of Tyr807.
Immunogen Range
776-825
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:40000
Specificity
BL-CAM (Phospho-Tyr807) Antibody detects endogenous levels of BL-CAM only when phosphorylated at Tyr807.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CD22
Database Links
Gene ID: 933 Swiss-Prot #: P20273
OMIM #: 107266 Unigene #: Hs.709215
Target Modification
Phospho
Modification Sites
H:Y807
Application Images
Image 1
Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using BL-CAM (Phospho-Tyr807) Antibody. The lane on the right is blocked with the phospho peptide.