The antiserum was produced against synthesized peptide derived from human Ephrin B1/B2 around the phosphorylation site of Tyr329.
Immunogen Range
295-344
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Ephrin B1/B2 (Phospho-Tyr329) Antibody detects endogenous levels of Ephrin B1/B2 only when phosphorylated at Tyr329.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2 (Phospho-Tyr329) Antibody. The picture on the right is blocked with the phospho peptide.