The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
WEE1 (Phospho-Ser53) Antibody
Synonyms
WEE1hu; Wee1-like protein kinase; kinase WEE1
Immunogen
The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser53.
Immunogen Range
19-68
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:20000
Specificity
WEE1 (Phospho-Ser53) Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser53.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
WEE1
Database Links
Gene ID: 7465 Swiss-Prot #: P30291
OMIM #: 193525 Unigene #: Hs.249441
Target Modification
Phospho
Modification Sites
H:S53 M:S52 R:S52
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WEE1 (Phospho-Ser53) Antibody. The picture on the right is blocked with the phospho peptide.