The antiserum was produced against synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/S218/232.
Immunogen Range
191-240
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:40000
Specificity
RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/S218/232.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody. The lane on the right is blocked with the phospho peptide.