The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
C-RAF (Phospho-Thr269) Antibody
Synonyms
C-RAF; C-Raf; CRAF; kinase Raf1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; Raf proto-oncogene serine/threonine-protein kinase; RAF-1; Raf-1; RAF1; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
Immunogen
The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Thr269.
Immunogen Range
231-280
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:40000
Specificity
C-RAF (Phospho-Thr269) Antibody detects endogenous levels of C-RAF only when phosphorylated at Thr269.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RAF1
Database Links
Gene ID: 5894 Swiss-Prot #: P04049
OMIM #: 164760/611553/611554 Unigene #: Hs.159130
Target Modification
Phospho
Modification Sites
H:T269 M:T269 R:T269
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using C-RAF (Phospho-Thr269) Antibody. The picture on the right is blocked with the phospho peptide.