The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
PKR (Phospho-Thr258) Antibody
Synonyms
Interferon-inducible RNA-dependent protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Protein kinase RNA-activated; PKR; p68 kinase; P1/eIF-2A protein kinase
Immunogen
The antiserum was produced against synthesized peptide derived from human PKR around the phosphorylation site of Thr258.
Immunogen Range
226-275
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
PKR (Phospho-Thr258) Antibody detects endogenous levels of PKR only when phosphorylated at Thr258.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
E2AK2
Database Links
Gene ID: 5610 Swiss-Prot #: P19525
OMIM #: 176871 Unigene #: Hs.131431
Target Modification
Phospho
Modification Sites
H:T258
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKR (Phospho-Thr258) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKR (Phospho-Thr258) Antibody. The lane on the right is blocked with the phospho peptide.