The antiserum was produced against synthesized peptide derived from human PAK3 around the phosphorylation site of Ser154.
Immunogen Range
121-170
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
PAK3 (Phospho-Ser154) Antibody detects endogenous levels of PAK3 only when phosphorylated at Ser154.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PAK3
Database Links
Gene ID: 5063 Swiss-Prot #: O75914
OMIM #: 300142/300558 Unigene #: Hs.656789
Target Modification
Phospho
Modification Sites
H:S154 M:S154 R:S139
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PAK3 (Phospho-Ser154) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from rat heart, using PAK3 (Phospho-Ser154) Antibody. The lane on the right is blocked with the phospho peptide.