The antiserum was produced against synthesized peptide derived from human PAK2 around the phosphorylation site of Ser141.
Immunogen Range
107-156
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
PAK2 (Phospho-Ser141) Antibody detects endogenous levels of PAK2 only when phosphorylated at Ser141.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PAK2
Database Links
Gene ID: 5062 Swiss-Prot #: Q13177
OMIM #: 605022 Unigene #: Hs.518530
Target Modification
Phospho
Modification Sites
H:S141 M:S141 R:S141
Application Images
Image 1
Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 24h/Jurkat cells treated with PMA 125ng/ml 30', using PAK2 (Phospho-Ser141) Antibody. The lane on the right is blocked with the phospho peptide.