The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase beta around the phosphorylation site of Ser423.
Immunogen Range
389-438
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
p70 S6 Kinase beta (Phospho-Ser423) Antibody detects endogenous levels of p70 S6 Kinase beta only when phosphorylated at Ser423.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RPS6KB2
Database Links
Gene ID: 6199 Swiss-Prot #: Q9UBS0
OMIM #: 608939 Unigene #: Hs.534345
Target Modification
Phospho
Modification Sites
H:S423 M:S423
Application Images
Image 1
Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 5', using p70 S6 Kinase beta (Phospho-Ser423) Antibody. The lane on the right is blocked with the phospho peptide.