The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Ser446.
Immunogen Range
411-460
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
B-RAF (Phospho-Ser446) Antibody detects endogenous levels of B-RAF only when phosphorylated at Ser446.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human brain, using B-RAF (Phospho-Ser446) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using B-RAF (Phospho-Ser446) Antibody. The lane on the right is blocked with the phospho peptide.