The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Thr142.
Immunogen Range
108-157
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:20000
Specificity
HSF1 (Phospho-Thr142) Antibody detects endogenous levels of HSF1 only when phosphorylated at Thr142.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HSF1
Database Links
Gene ID: 3297 Swiss-Prot #: Q00613
OMIM #: 140580 Unigene #: Hs.530227/Hs.571397
Target Modification
Phospho
Modification Sites
H:T142 M:T142
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using HSF1 (Phospho-Thr142) Antibody. The picture on the right is blocked with the phospho peptide.