The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Ser1071.
Immunogen Range
1036-1085
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
EGFR (Phospho-Ser1071) Antibody detects endogenous levels of EGFR only when phosphorylated at Ser1071.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EGFR
Database Links
Gene ID: 1956 Swiss-Prot #: P00533
OMIM #: 131550/211980 Unigene #: Hs.488293
Target Modification
Phospho
Modification Sites
H:S1071 M:S1071 R:S1070
Application Images
Image 1
Western blot analysis of lysates from HepG2 cells treated with serum 20% 15', using EGFR (Phospho-Ser1071) Antibody. The lane on the right is blocked with the phospho peptide.