The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
14-3-3 beta/zeta (Phospho-Ser184/186) Antibody
Synonyms
14-3-3 protein zeta/delta; 1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Protein kinase C inhibitor protein-1; YWHAZ
Immunogen
The antiserum was produced against synthesized peptide derived from human 14-3-3 beta/zeta around the phosphorylation site of Ser184/186.
Immunogen Range
151-200
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
14-3-3 beta/zeta (Phospho-Ser184/186) Antibody detects endogenous levels of 14-3-3 beta/zeta only when phosphorylated at Ser184/186.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of lysates from RAW264.7 cells treated with UV 15', using 14-3-3 beta/zeta (Phospho-Ser184/186) Antibody. The lane on the right is blocked with the phospho peptide.