The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr739.
Immunogen Range
706-755
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
SP1 (Phospho-Thr739) Antibody detects endogenous levels of SP1 only when phosphorylated at Thr739.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SP1
Database Links
Gene ID: 6667 Swiss-Prot #: P08047
OMIM #: 189906 Unigene #: Hs.620754/Hs.649191
Target Modification
Phospho
Modification Sites
H:T739 M:T738 R:T740
Application Images
Image 1
Western blot analysis of lysates from COS7 cells treated with serum 20% 15', using SP1 (Phospho-Thr739) Antibody. The lane on the right is blocked with the phospho peptide.