The antiserum was produced against synthesized peptide derived from human SEK1/MKK4 around the phosphorylation site of Ser80.
Immunogen Range
46-95
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
SEK1/MKK4 (Phospho-Ser80) Antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at Ser80.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP2K4
Database Links
Gene ID: 6416 Swiss-Prot #: P45985
OMIM #: 601335 Unigene #: Hs.514681
Target Modification
Phospho
Modification Sites
H:S80 M:S78 R:S78
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SEK1/MKK4 (Phospho-Ser80) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells and NIH/3T3 cells, using SEK1/MKK4 (Phospho-Ser80) Antibody. The lane on the right is blocked with the phospho peptide.