The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Ser411.
Immunogen Range
401-450
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
p70 S6 Kinase (Phospho-Ser411) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Ser411.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RPS6KB1
Database Links
Gene ID: 6198 Swiss-Prot #: P23443
OMIM #: 608938 Unigene #: Hs.463642
Target Modification
Phospho
Modification Sites
H:S434 M:S434 R:S434
Application Images
Image 1
Western blot analysis of lysates from K562 cells, NIH/3T3 cells and COLO205 cells, using p70 S6 Kinase (Phospho-Ser411) Antibody. The lane on the right is blocked with the phospho peptide.