The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
p38 MAPK (Phospho-Tyr182) Antibody
Synonyms
protein; kinase p38-alpha; MAP kinase MXI2; MAP kinase p38; MAPK14; MAX-interacting protein 2; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38; MK14; MXI2
Immunogen
The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Tyr182.
Immunogen Range
147-196
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
p38 MAPK (Phospho-Tyr182) Antibody detects endogenous levels of p38 MAPK only when phosphorylated at Tyr182.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAPK14
Database Links
Gene ID: 1432 Swiss-Prot #: Q16539
OMIM #: 600289 Unigene #: Hs.485233/Hs.588289
Target Modification
Phospho
Modification Sites
H:Y182 M:Y182 R:Y182
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p38 MAPK (Phospho-Tyr182) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from RAW264.7 cells and 293 cells, using p38 MAPK (Phospho-Tyr182) Antibody. The lane on the right is blocked with the phospho peptide.