The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser932.
Immunogen Range
899-948
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
NF-kappaB p105/p50 (Phospho-Ser932) Antibody detects endogenous levels of NF-kappaB p105/p50 only when phosphorylated at Ser932.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NFKB1
Database Links
Gene ID: 4790 Swiss-Prot #: P19838
OMIM #: 164011 Unigene #: Hs.654408
Target Modification
Phospho
Modification Sites
H:S932 M:S935 R:S486
Application Images
Image 1
Western blot analysis of lysates from HeLa cells treated with CA+TNF 20ng/ml 10', using NF-kappaB p105/p50 (Phospho-Ser932) Antibody. The lane on the left is blocked with the phospho peptide.