The antiserum was produced against synthesized peptide derived from human Merlin around the phosphorylation site of Ser518.
Immunogen Range
485-534
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:4000
Specificity
Merlin (Phospho-Ser518) Antibody detects endogenous levels of Merlin only when phosphorylated at Ser518.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NF2
Database Links
Gene ID: 4771 Swiss-Prot #: P35240
OMIM #: 101000/162091/607379 Unigene #: Hs.187898
Target Modification
Phospho
Modification Sites
H:S518 M:S518 R:S514
Application Images
Image 1
Western blot analysis of lysates from HUVEC cells treated with IFN-alpha 1000U/ml 18h, using Merlin (Phospho-Ser518) Antibody. The lane on the right is blocked with the phospho peptide.