The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser221.
Immunogen Range
193-242
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
MEK1/2 (Phospho-Ser221) Antibody detects endogenous levels of MEK1/2 only when phosphorylated at Ser221.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser221) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells treated with serum 20% 15' and Jurkat cells treated with EGF, using MEK1/2 (Phospho-Ser221) Antibody. The lane on the right is blocked with the phospho peptide.