The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
JAK2 (Phospho-Tyr221) Antibody
Synonyms
JAK-2; JAK2; Janus kinase 2; kinase Jak2
Immunogen
The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr221.
Immunogen Range
191-240
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
JAK2 (Phospho-Tyr221) Antibody detects endogenous levels of JAK2 only when phosphorylated at Tyr221.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JAK2 (Phospho-Tyr221) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells, using JAK2 (Phospho-Tyr221) Antibody. The lane on the right is blocked with the phospho peptide.